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Easytaq

WebDNA 聚合酶(EasyTaq、FastPfu 等)、电泳凝胶回收 试剂盒、酵母基因组抽提试剂盒等均购自 TransGen Biotech 公司。 主要仪器 电泳凝胶成像系统、BioRad 基因枪、PCR 仪、微量分光光度计等仪器 设备均由本实验室提供。 1.2 实验方法 生物信息学分析 从隐球 … WebEasyTaq PCR Supermix DNA聚合酶、pEASY-Blunt Simple Cloning Vector购自全式金公司;其他化学试剂购自Sigma公司。 2 结果与分析 2.1 拟南芥cpk6突变体纯合鉴定 为了获得纯合突变体,用SDS方法提取Col-0和cpk6幼嫩叶子的总DNA,利用T-DNA特异引物LBb1.3和CPK6基因特异引物LP和RP进行PCR ...

EasyTaq® DNA Polymerase for PAGE - TransGen Biotech

WebEasyTaq ® PCR SuperMix is a ready-to-use mixture of EasyTaq ® DNA Polymerase, dNTPs, and optimized reaction buffer at a concentration of 2×. For DNA amplification, simply add the template, primers, and water to allow the SuperMix solution to react at a concentration of 1×. For 2×EasyTaq ® PCR SuperMix (-dye), the PCR products which … Web请教论坛里的各位高手:我的目的片段长度是2.5kb多一点,是从基因组中扩增。用全式金的EasyTaq酶可以得到很好的目的条带,而我用pfu(promega)没有目的条带。想请高人帮我分析分析 french singular pronouns https://htawa.net

EasyTaq® DNA Polymerase - genewiz.com

WebJan 8, 2024 · 3.根据权利要求1所述用于牛个体识别和亲子鉴定的微卫星标记引物组12-plex快速检测方法,其特征在于:所述pcr扩增得到12条目标微卫星序列产物,按照如下步骤进行:牛的微卫星12重pcr扩增:使用上述12对荧光标记引物,按照各自确定的引物终浓度,配成 … Web3 Posts - See Instagram photos and videos from 'easytaq' hashtag WebMar 28, 2024 · 2×EasyTaq® PCR SuperMix EasyTaq® PCR SuperMix for PAGE is a ready-to-use mixture of EasyTaq® DNA Polymerase for PAGE, dNTPs and optimized buffer. The SuperMix for PAGE is provided at 2× concentration and used at 1× concentration by adding template, primers and H2O. • Extension rate is about 1-2 kb/min. • Unique … fast relief from acidity

2×EasyTaq® PCR SuperMix - TransGen Biotech

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Easytaq

TransGen Biotech EasyTaq PCR SuperMix - Alibaba

WebSpecifications and pricing of commercially available Taq DNA Polymerases and High-Fidelity DNA Polymerases in Canada. Download this PDF Table Max price per 50 ul reaction is based on the list price (CAD) of the … Web10×EasyTaq® Buffer provides the preferred pH and ionic strength for PCR amplification reactions performed with EasyTaq® DNA Polymerase. Features • Standalone buffer. • …

Easytaq

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EasyTaq® DNA Polymerase is purified from E. coli expressing a cloned DNA polymerase from Thermus aquaticus. The enzyme consists of a single polypeptide with a molecular weight of approximately 94 kDa. EasyTaq® DNA Polymerase has 5′-3′ DNA polymerase activity and 5′-3′ exonuclease activity. WebThe 2x EasyTaq PCR SuperMix is a ready-to-use mixture of EasyTaq® DNA Polymerase, dNTPs and optimized buffer. The supermix is provided at 2x concentration and used at …

WebIn this study, an easy, rapid, reliable, and cost-effective colony polymerase chain reaction (PCR) method for screening mushroom transformants was developed: picking up a suitable amount of transformant’s tissue (1–10 μg) to 20 μl 0.25% Lywallzyme solution, and vortexing for 10 s followed by incubation at 34 °C for 15 min. http://www.life-tech.org/2xeasytaq-pcr-supermix/

WebMar 28, 2024 · EasyTaq ® PCR SuperMix for PAGE is a ready-to-use mixture of EasyTaq ® DNA Polymerase for PAGE, dNTPs and optimized buffer. The SuperMix for PAGE is provided at 2× concentration and used at 1× concentration … WebEasyTaq ® PCR SuperMix is a ready-to-use mixture of EasyTaq ® DNA Polymerase, dNTPs, and optimized reaction buffer at a concentration of 2×. For DNA amplification, …

WebOct 14, 2024 · dNTPs、Easytaq®DNA polymerase for PAGE等试剂均来自北京全式金生物技术(TransGen Biotech)有限公司。 用于丙烯酰胺凝胶电泳的AgNO3、NaOH等试剂均购自北京鼎国生物科技公司。

WebEasyTaq® DNA Polymerase for PAGE is purified from E. coliexpressing a cloned DNA polymerase from Thermus aquaticus. The enzyme consists of a single polypeptide with a molecular weight of approximately 94 kDa. … fast relief for sinus headacheWebJan 28, 2024 · Engineering transporter for improved efflux efficiency provides a powerful strategy to alleviate product-associated cytotoxicity and promote microbial production of desired compounds. However, the scarcity of efficient transporters limits its application in the construction of microbial cell factories. fast relief for uti painWebSep 3, 2015 · PCR 扩增体系为25 一,包含12.5 结果与分析 EasyTaq PCR SuperMix,1 p6,F1/RI(10斗moL/L),1l 因组DNA,9.5山ddH20。 扩增程序为:94 oC 预变性5 min; 000bp 的单一条带(图1A),经条带回收、纯化后连 接到克 94变性40 S,56退火45 S,72 oC 延伸1 min,35 个循环; 隆载体上,通过 ... fast relief for severe heartburnWebEasyTaq® DNA Polymerase (with 2.5 mM dNTPs) AP111-11: 500 units: IGtechnology is an Egyptian company that provides science equipment and laboratory supplies to the scientific research community all over EGYPT. We collaborate with the leading manufacturers in the Chemicals supplies and laboratory equipment industry to bring you a host of ... french singular to plural rulesWeb10x EasyTaq Buffer (with Mg2+) – GK101. Only 60$ when you add 5 vials (6 ml total volume). $ 14.00 CAD. 0 in stock (expect 7-21 days for delivery) Format. Only 60$ when … fast relief for sinus infectionhttp://www.life-tech.org/2xeasytaq-pcr-supermix/ french sink cabinetWebThe arrows point to the specific amplicons that were then extracted ad purified using the Favorprep GEL/PCR Purification Kit. Contact us for your design! Successful PCR Overlap Assembly with FastPfu SUCCESS ! … french sink fountain